The event was scheduled for Sunday, March 2 and was officially called off on the evening of Wednesday, February 26.
Event organiser Steven Fehring said he was devastated the event could not go ahead.
“It was going so well and looking like a really positive year. There was lots of good registers, and a lot of interest in it,” he said.
Mr Fehring said the traffic management team he was working with informed him the new VicRoads regulations called for a significant increase in staff and infrastructure.
“Every road that came on to a road that we were closing had to have a traffic controller on,” he said.
“We were going from having two people at the start and finish of the road closure to 20 people across the whole thing.”
Along with higher labour costs, Mr Fehring said more expensive barriers would also need to be hired.
Given the major cost increase, the fundraising aspect of the event for Cohuna District Hospital would have become unfeasible.
“We tried other options — halving the course length, only having half the course, and changing up a few things — and even that meant the hospital got nothing out of the fundraising,” Mr Fehring said.
Unfortunately, the future of the event is now uncertain unless the costs associated changed.
A spokesperson for VicRoads said traffic management requirements were the same across the country, and laws had been reformed nationwide over the past few years.
A raffle for the hospital will still be held on Sunday, and Mr Fehring said those registered for the event could choose to donate their entry fee and run the course virtually.